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. 2017 Feb 17;12(2):e0172371. doi: 10.1371/journal.pone.0172371

Table 5. Antibody concentrations used for flow cytometry analysis of multidrug resistance proteins expressed by endothelial cells.

Anti-MDR1 Anti-MRP1 Anti-MRP4 Anti- MRP5 Anti -BCRP
1 st Ab MDR1/1 MDR1/2 MDR1/3 MRP1/1 MRP1/2 MRP4/1 MRP4/2 MRP5/1 BCRP/1 BCRP/2
1:50 1:50 1:5000 1:50 1:10000 1:100 1:100 1:100 1:50 1:100
2nd Ab FITC anti-mouse FITC anti-mouse Alexa488 anti-rat FITC anti-rabbit Alexa488 anti-rat FITC anti-mouse
1:200 1:200 1:200 1:200 1:200 1:200