Table 5. Antibody concentrations used for flow cytometry analysis of multidrug resistance proteins expressed by endothelial cells.
Anti-MDR1 | Anti-MRP1 | Anti-MRP4 | Anti- MRP5 | Anti -BCRP | ||||||
---|---|---|---|---|---|---|---|---|---|---|
1 st Ab | MDR1/1 | MDR1/2 | MDR1/3 | MRP1/1 | MRP1/2 | MRP4/1 | MRP4/2 | MRP5/1 | BCRP/1 | BCRP/2 |
1:50 | 1:50 | 1:5000 | 1:50 | 1:10000 | 1:100 | 1:100 | 1:100 | 1:50 | 1:100 | |
2nd Ab | FITC anti-mouse | FITC anti-mouse | Alexa488 anti-rat | FITC anti-rabbit | Alexa488 anti-rat | FITC anti-mouse | ||||
1:200 | 1:200 | 1:200 | 1:200 | 1:200 | 1:200 |