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. 2017 Feb 17;12(2):e0172630. doi: 10.1371/journal.pone.0172630

Table 1. Expected fragment sizes of verification PCRs.

Locus Forward primer Reverse primer Fragment WT [bp] Fragment after deletion [bp]
aer Aer-Delcheck-for2 McpC-Delcheck-rev 2605 1370
tcp Tcp-Delcheck-for Tcp-Delcheck-rev 2150 509
tsr Tsr-Delcheck-for Tsr-Delcheck-rev 2203 544
trg Trg-Delcheck-for Trg-Delcheck-rev 2153 530
cheM (tar) CheM-Delcheck-for CheM-Delcheck-rev 2555 896
mcpC McpC-Delcheck-for McpC-Delcheck-rev 2330 768
mcpB McpB-Delcheck-for2 McpB-Delcheck-rev 2414 813