Table 1. Expected fragment sizes of verification PCRs.
Locus | Forward primer | Reverse primer | Fragment WT [bp] | Fragment after deletion [bp] |
---|---|---|---|---|
aer | Aer-Delcheck-for2 | McpC-Delcheck-rev | 2605 | 1370 |
tcp | Tcp-Delcheck-for | Tcp-Delcheck-rev | 2150 | 509 |
tsr | Tsr-Delcheck-for | Tsr-Delcheck-rev | 2203 | 544 |
trg | Trg-Delcheck-for | Trg-Delcheck-rev | 2153 | 530 |
cheM (tar) | CheM-Delcheck-for | CheM-Delcheck-rev | 2555 | 896 |
mcpC | McpC-Delcheck-for | McpC-Delcheck-rev | 2330 | 768 |
mcpB | McpB-Delcheck-for2 | McpB-Delcheck-rev | 2414 | 813 |