. 2017 Feb 17;12(2):e0172630. doi: 10.1371/journal.pone.0172630

Table 4. Plasmids used in this study.

Plasmid	Relevant characteristic(s)	Source or Reference
pDL1098	Temperature-sensitive mTn10 delivery vector, Cm^r, Sp^r	[39]
pKD4	aph resistance cassette flanked by FRT sites, λ Pir dependent replication, Km^r, Ap^r	[36]
pSIM5	temperature-sensitive replication (30°C) and Red recombinase expression (42°C), Cm^r	[38]
pWRG99	pKD46 [36] derivative, temperature-sensitive replication (30°C), arabinose-inducible expression of Red recombinase, Tet-inducible expression of I-SceI, Ap^r	[4]
pWRG717	pBluescript II SK+ derivative, aph resistance cassette and I-SceI cleavage site, Km^r, Ap^r	This study
pWRG730	pSIM5 [38] derivative, temperature-sensitive replication (30°C) and Red recombinase expression (42°C), Tet-inducible expression of I-SceI, Cm^r	This study
pWRG829	pBluescript II SK+ derivative, aad9 resistance cassette and I-SceI cleavage site, Sp^r, Ap^r	This study
pWRG832	pWRG717 derivative, aph resistance cassette reversed, Km^r, Ap^r	This study
pWRG841	P_cheM::cheM in pCRII-TOPO, Km^r, Ap^r	This study
pWRG847	pCheM; P_cheM::cheM in pWSK29, Ap^r	This study
pWRG865	pWRG829 derivative, aad9 resistance cassette reversed, Sp^r, Ap^r	This study
pWSK29	Low-copy-number vector, Ap^r	[53]