FIG. 2.

Endometriosis dysregulates liver gene expression. RNA was extracted from liver tissue collected from sham surgery mice or mice in which endometriosis was induced. A) Real-time qPCR results shows the altered liver gene expression as identified in the array. The levels of Cyp2r1, Fabp4, Mrc1, and Rock2 expression were increased, whereas those of Mmd2 and Igfbp1 expression were decreased. Data are relative fold-change expression in mice with endometriosis (E) compared to sham surgery controls (C). The expression levels of all genes were normalized to those of β-actin. Bars in each graph are mean ± SEM of two individual experiments, each performed in triplicate (n = 16 mice per group). *Statistical significance (P < 0.05). B) Western blot analysis shows the protein levels. GADPH was used as a protein loading control. Liver protein (25 μg) was subjected to 4%–12% SDS-PAGE and immunoblotting. The protein product levels of Cyp2r1, Fabp4, Mrc1, and Rock2 genes were increased, whereas those of Igfbp1, Mmd2 were decreased. In addition to those genes identified in the array, we showed the increased expression of mRNA levels of the Lep and Pparg genes in real-time qPCR (C) and increased protein levels by Western blot analysis (D) in endometriosis (E), compared to those in controls (C).