FIG. 3.

Decreased cell viability is associated with ER stress in the absence of EIF2AK3. Colorimetric MTT assay demonstrated decreased numbers of viable cells in the TM-treated EIF2AK3 null hTERT-HM cell; 24 and 36 h post-TM exposure, cell viability had decreased by approximately 18% and 58%, respectively, in comparison to U-, M-, or S-treated hTERT-HM's and those cells not exposed to TM. All experiments were performed in triplicate (n = 3). Student t-test was used for statistical comparison between the S and Pi ± TM samples. ***P < 0.0001