Figure 8. GMR58H01 neurons elicit labella spreading and proboscis retraction.
(A,B) Artificial activation of GMR58H01 neurons using TrpA1. Heat induced activation elicits labella spreading (middle panel (A)) and leads to the retraction of the proboscis (middle panel (B), arrow). At the control temperature before (left panels) and after (right panels) activation the proboscis is retracted. Insets in (A) show magnifications of the labella and the double arrow indicates the spreading of the labella. (C) Artificially activation of GMR58H01 neurons using Chrimson while evoking sucrose induced proboscis extension. At blue light (control), flies show full PER upon 200 mM sucrose stimulation (left panel). Red light activation results in labella spreading and proboscis extension (middle panel). Red light activation during 200 mM sucrose stimulation prevents proboscis extension (right panel). (D) Expression pattern of GMR58H01 in the adult central brain. Arrow points to the axons that are leaving the brain. (E) Whole head preparation of GMR58H01>mCD8-GFP flies (left panel) reveals that the identified MNs (green) innervate muscle 1, 4, and haustellum muscles (asterisk). The side view of the proboscis (right panels) shows innervation of muscles 7 and 8 in the haustellum. Muscles are visualized by the F-actin marker phalloidin (blue). (F) Schematic drawing of the head muscles with innervated muscles highlighted in blue. Scale bars, 50 µm. (G) Quantification of the behavioral phenotypes in control and experimental animals. Numbers and significances are listed in Supplementary file 1. See also Figure 8—figure supplement 1, Videos 14, 15 and 16.
Figure 8—figure supplement 1. VT020958 neurons elicit labella spreading.
