Table 1. Specific activities of cell extracts from different denitrifying bacteria grown with PA or B.
| Organism | Growth substrate | Substrates in enzyme assay | Succinyl-CoA consumption (nmol min−1 mg−1)a | CoA formation (nmol min−1 mg−1)a | Benzoyl-CoA formation (nmol min−1 mg−1) | Phthalate consumption (nmol min−1 mg−1) |
|---|---|---|---|---|---|---|
| T. chlorobenzoica 3CB-1 | PA | PA | n.d. | n.d. | n.d. | ⩽0.3 |
| T. chlorobenzoica 3CB-1 | PA | Succinyl-CoA PA | 31±9 | 4±1 | 13±2 | 34±9 |
| ‘A. aromaticum' EbN1 | PA | Succinyl-CoA PA | 26±3 | 3±1 | 12±3 | n.d. |
| A. evansii KB740 | PA | Succinyl-CoA PA | 18±3 | 3±1 | 6±1 | n.d. |
| T. chlorobenzoica 3CB-1 | B | Succinyl-CoA PA | n.d. | n.d. | <0.1 | ⩽0.8 |
| T. chlorobenzoica 3CB-1 | PA | Succinyl-CoA B | ⩽2 | ⩽1 | <0.1 | n.d. |
| T. chlorobenzoica 3CB-1 | PA | CoA/MgATP B | n.d. | n.d. | <0.1 | n.d. |
Abbreviations: B, benzoate; n.d., not determined; PA, pthalate. Errors are given as s.d.s of the mean of three biological replicates.
a
After subtraction of PA-independent succinyl-CoA consumption.