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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Pain. 2016 Dec;157(12):2722–2730. doi: 10.1097/j.pain.0000000000000692

Figure 2.

Figure 2

Dural application of IL-6 primes animals to sub-threshold stimuli. Animals that received a prior application of IL-6 exhibit cutaneous allodynia when dural pH 6.8 (A, n=9 for all groups ) or pH 7.0 (B, n=10 for all groups) is applied after the resolution of allodynia at 72 hours. Withdrawal thresholds to tactile stimuli applied to the face (A & B) were measured in animals after application of dural pH 6.8 or pH 7.0 72 hours post IL-6. Significant differences among means for each group were determined by analysis of variance followed by Bonferroni post hoc test. Increasing the pH value to pH 7.2 did not produce significant facial allodynia in either IL-6 or vehicle-treated rats (C). ASIC3 blocker APETx2 was able to attenuate facial (C) and hindpaw (D) allodynia when administered with pH 6.8 at 72 hours post IL-6. Stimulation of the dura with Vehicle at 72 hours post IL-6 did not produce facial allodynia (C). Significant (**p < 0.01) differences among change of means at 72 hours for each group were determined using a t-test (C & D). (A) Facial: time F (2, 36) = 26.53, P <0.0001, treatment F (1, 87) = 0.0046,P =0.0001; (B) Facial: time F (2, 36) = 5.112, P =0.0111, treatment F (1, 36) = 19.70, P < 0.0001.