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. 2017 Feb 17;12(2):e0172125. doi: 10.1371/journal.pone.0172125

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© 2017 Arsic et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (a) Immunoblot analysis showing the specific co-immunoprecipitation of MYC-tagged delta133p53ß and endogenous RhoB or RhoC, to a lower extent, but not RhoA. (b) Immunoblot analysis showing the co-immunoprecipitation of endogenous RhoB and delta133p53ß with anti-RhoB antibodies. Arrow shows co-immunoprecipitation of delta133p53ß isoform. (c) In-vitro binding assay showing the direct interaction between recombinant RhoB-GST fusion protein and delta133p53ß, but not α or γ. (d) Cellular fractionation showing RhoB cytoplasmic re-localization in SW620 cells transfected with shRNAS against delta133p53 compared with shControl (mock-transfected cells). (e) Immunofluorescence analysis of RhoB localization in control SW620 cells (shControl) or after transfection with shdelta133p53. Arrows indicate examples of RhoB cytoplasmic localization. Scale bar: 10μm. (f) Confocal images showing the co-localization of RhoB and delta133p53ß in the nucleus of SW480 cells that overexpress delta133p53ß. Arrows indicate examples co-localization of RhoB and delta133p53ß in the nucleus. Scale bar: 10μm.