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. 2016 Oct 4;6(10):e910. doi: 10.1038/tp.2016.181

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Copyright © 2016 The Author(s)

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Molecular mechanisms are involved in the impaired GABA synthesis, uptake and release in the medial prefrontal cortices (mPFCs) of chronic unpredictable mild stress (CUMS)-induced depression mice. (a, b) The expression and relative quantity of proteins glutamate decarboxylase-67 (GAD-67), vesicular GAT (VGAT) and GABA transporter (GAT-3) were studied by western blot. (a) The expressions of GAD-67, VGAT and GAT-3 from a control mouse versus a CUMS-induced depression mouse, where internal control is performed with β-actin. (b) The normalized ratios of proteins (GAD-67, VGAT and GAT-3) to β-actin from control mice (dark-gray bars, n=8) versus CUMS-induced depression mice (light-gray bars, n=8). The relative ratios for control mice are normalized to be one. (c, d) The expressions and the relative quantity of messenger RNAs (mRNAs) for GAD-67, VGAT and GAT-3 were studied using high-throughput sequencing and quantitative real-time RT-PCR (qRT-PCR). (c) The relative values of mRNAs for GAD-67, VGAT and GAT-3 in control mice (dark-gray bars, n=8) and CUMS-induced depression mice (light-gray bars, n=8), which are analyzed using high-throughput mRNA sequencing. The relative expression levels of mRNAs in CUMS-induced depression mice are normalized to that in controls based on NOIseq package method. Diverge probability larger than 0.8 is thought as significant difference (#P⩾0.8). (d) The relative values of mRNAs for GAD-67, VGAT and GAT-3 in control mice (dark-gray bars, n=8) versus CUMS-induced depression mice (light-gray bars, n=8), which are carried out by qRT-PCR. β-actin was set as internal control. The relative values for control mice were normalized to be one. (e, f) The expressions and the relative quantity of mRNAs for miRNA-15b-5p, miRNA-144-3p, miRNA-582-5p and miRNA-879-5p were studied using high-throughput sequencing and qRT-PCR. (e) The relative values of miRNA-15b-5p, miRNA-144-3p, miRNA-582-5p and miRNA-879-5p from control mice (dark-gray bars, n=8) versus CUMS-induced depression mice (light-gray bars, n=8), which are analyzed using high-throughput miRNA sequencing. (f) The relative values of miRNA-15b-5p, miRNA-144-3p, miRNA-582-5p and miRNA-879-5p from control mice (dark-gray bars, n=8) versus CUMS-induced depression mice (light-gray bars, n=8), which are studied by qRT-PCR of miRNAs. U6 was set to be internal control. The relative values for control mice were normalized to be one.*P<0.05, **P<0.01 and ***P<0.001.