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. 2016 Oct 4;6(10):e910. doi: 10.1038/tp.2016.181

Figure 5.

Figure 5

The miRNAs targeted messenger RNAs (mRNAs) for GABA synthesis and transporters are validated by Luciferase reporter assay. (a–c) Luciferase reporter assay is performed by the co-transfection of luciferase reporter containing wild or mutant 3′-untranslated repeat (UTR) of GABA transporter (GAT-3) mRNA with miRNA-15b-5p, miRNA-879-5p and miR-144-3p mimic or their negative control (NC) into HEK293T cells. (d, e) Luciferase reporter assay is performed by the co-transfection of luciferase reporter containing the wild or mutant 3′-UTR of vesicular GAT (VGAT) mRNA with miR-582-5p and miR-144-3p mimic or their NC into HEK293T cells. (f) Luciferase reporter assay is performed by the co-transfection of luciferase reporter containing the wild or mutant 3′-UTR of GAD1 mRNA with miR-144-3p mimic or their NC into HEK293T cells. Luciferase activity was determined 48 h after co-transfection. **P<0.01 and ***P<0.001.