Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 18;6(10):e918. doi: 10.1038/tp.2016.200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Kynurenine 3-monooxygenase (KMO) and 3-hydroxyanthranilic acid dioxygenase (HAAO) transgene construct. (a) The KMO transgene vector was designed with a neomycin (neo) selection cassette to test for site-specific integration of the transgene. Proper incorporation of the transgene results in gene-trapping between the reporter gene (lacZ) from the cassette and the Kmo gene during transcription. When these transcripts become spliced together, an insertion mutation is created resulting in a non-functional KMO protein and a KMO^−/− mouse. The vector also includes recombination sites (Flp recombination target (FRT), loxP) that can be used to create a conditional-ready mouse targeted for the gene of interest (that is, KMO-floxed). (b) The presence of the transgene in KMO transgenic mice were confirmed using RT-PCR for either the wild-type allele (476-bp band) or the transgenic allele (592-bp band), as indicated in a. (c) The HAAO transgene vector was designed identical to the KMO transgene (a), targeting the HAAO genetic sequence. (d) The presence of the HAAO transgene was confirmed in the same manner as the KMO transgene using a RT-PCR reaction for the wild-type allele (516 bp) and for the transgenic allele (594 bp).