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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Pathol. 2017 Jan 6;241(4):501–510. doi: 10.1002/path.4854

Figure 3.

Figure 3

Mdm4 overexpression decreases p53 Loss of Heterozygosity (LOH) in Mdm4Tg6 p53R172H/+ and Mdm4Tg15 p53R172H/+ tumours. (A) The expression levels of Mdm4 were higher in the double mutant tumours as determined by RT-qPCR. (B) Representative IHC staining for Mdm4 and p53 in Mdm4Tg6 p53R172H/+ tumours with or without p53 LOH. (C) Decreased frequency of p53 LOH in double mutant tumours. (D) Overexpression of Mdm4 in H318-1 osteosarcoma cell line repressed the expression of mutant p53 target genes (E). *, ** and *** indicate p <0.05, p <0.01 and p < 0.001, respectively.