FIG. 5.

In cellulo and in vivo nonlinear imaging of hGVs at 18 MHz. (a)–(d) In cellulo images of hGVs in Xenopus laevis oocytes. (a) B-Mode imaging of 5 oocytes. The first three oocytes, labelled with white arrows, were injected with hGVs (50 nl, 1.8 nM). (b) Corresponding amplitude modulation image. (c) and (d) Images of the same sample after collapsing hGVs. (e)–(h) In vivo imaging of a wild-type mouse after hGVs were introduced into its colon. Left, B-Mode images before (top) and after (bottom) collapse. Right, AM images before (top) and after (bottom) collapse. Scale bars represent 1 mm. Oocytes and hGVs were imaged at a depth of 8 mm.