Fig. 7.

Expression pattern of CK7, CK19, p16, and HR HPV in squamous cell carcinoma (SCC). HE staining shows SCC with solid tumor nests (SCC#8) (a), SCC with cystic change (SCC#19) (b), and SCC with glandular differentiation (SCC#29) (c). CK7 staining is negative in SCC#8 (d) and diffusely positive in SCC#19 (e) and SCC#29 (f). CK19 staining pattern is patchy in SCC#8 (g) and SCC#19 (h), and diffuse in SCC#29 (i). p16 staining is diffusely positive with variable intensity in SCC#8 (j), SCC#19 (k), and SCC#29 (l). HPV is present in mixture of episomal and integrated form in SCC#8 (m) and predominant episomal form in the cystic tumor nest and cellular debris shedding within the cystic space of SCC#19 (n), and integrated form in SCC#29 (o). Original magnifications: (a), (c), (d), (f), (g), (i), (j), (l), (m), and (o) x400; (b), (e), (h), (k) and (n) x200