Fig. 1.

miR-335 was associated with osteosarcoma stem cells. a The relative expression of miR-335 were determined by qRT-PCR in CD117 and Stro-1 double positive (DP) cells and double negative (DN) cells. b miR-335 relative expression in sphere and monolayer culture conditions were examined. c The relative expression of miR-335 in the cisplatin resistant and control cells were tested. d The cells were divided into two groups according to their expression of miR-335. e A representative flow cytometric analyses of CD117+/Sro-1+ expression among miR-335-high and miR-335-low cells. f CD117+/Sro-1+ DP ratio in miR-335-low group was generally higher compared with miR-335-high in all three cell line. g Western-Blotting assay showed that POU5F1 and Sox2 expression were significantly decreased in miR-335-high compared with miR-335-low cells. h The ability to form sarcospheres was higher in miR-335-low compared with miR-335-high cells. All experiments were carried out at least triplicates and the data were presented as the mean ± SD. Student t test was performed to evaluate the difference. *P < 0.05; **P < 0.01