Fig. 2.

Downregulation of miR-335 enhanced osteosarcoma stem cell-like characteristics. a miR-335 antagonist increased CD117/Stro-1 double positive ratio in all three cell lines. b The ability of cells treated with anti-miR-335 to form sarcosphere was higher compared with pre-miR-335 and negative control cells. c Transwell invasion assay demonstrated that anti-miR-335 promoted cell invasion while pre-miR-335 inhibited it. All experiments were carried out at least triplicates and the data were presented as the mean ± SD. Student t test was performed to evaluate the difference. *P < 0.05; **P < 0.01