Fig. 4.

miR-335 decreased in vivo tumor formation by targeting CSCs. a Anti-miR-335 treatment promoted chemoresistance while pre-miR-335 enhanced sensitivity in all three cell lines. b Combination of cisplatin and pre-miR-335 orchestrated to reduce the tumor volume. c Pre-miR-335 treatment brought down the sarcosphere formation ability induced by cisplatin. All experiments were carried out at least triplicates and the data were presented as the mean ± SD. Student t test was performed to evaluate the difference. *P < 0.05; **P < 0.01