Fig. 2.

Analysis of expression and RNase activity of rSj CP1412. a Lane 1: expression products of E. coli BL21 carrying plasmid pET28a-Sj CP1412 without IPTG induction; Lane 2: expression products of E. coli BL21 carrying plasmid pET28a-Sj CP1412 with IPTG induction; Lane 3: supernatant with expression products of E. coli BL21 carrying plasmid pET28a-Sj CP1412; Lane 4, precipitate of expression products of E. coli BL21 carrying plasmid SjpET28a- CP1412; Lane M, standard protein molecular weight marker. b Lane 1: standard protein molecular weight marker; Lane 2: purified rSj CP1412 protein. c Lane 1: yeast tRNA; Lane 2: RNA incubated inwater bath at 37 °C without Sj CP1412; Lane 3: products of RNA digested by 1.25 μg rSj CP1412 protein; Lane 4: products of RNA digested by 2.5 μg rSj CP1412 protein; Lane 5: products of RNA digested by 5 μg rSj CP1412 protein; Lane 6: products of RNA digested by commercial RNase A; Lane 7: products of RNA digested by 1.25 μg rTg SAG1 protein; Lane 8: products of RNA digested by 2.5 μg rTg SAG1 protein; Lane 9: products of RNA digested by 5 μg rTg SAG1 protein