Fig. 4.

Che-1 modulates HIF-1α protein expression. a WB analysis with the indicated Abs of TCEs from HCT116 and HT29 cells transiently transfected with stealth siRNA negative control (siControl) or siRNA Che-1 (siChe-1) and exposed to hypoxia where indicated. b qRT–PCR performed in HCT116 cells transiently transfected and treated as in a. Values were normalized to RPL19 mRNA expression. Error bars represent the standard error of three different experiments. *P ≤ 0,02, **P ≤ 0.01. c WB analysis with the indicated Abs of TCEs from HCT116 cells transiently transfected as in a. d SIAH-2 expression levels are reported as FPKM reconstructed from Cufflinks RNA-Seq analysis from HCT116 transiently transfected with siControl or siChe-1 and exposed to hypoxia. e qRT–PCR performed as in b. Values were normalized to RPL19 mRNA expression. Error bars represent the standard error of three different experiments. *P ≤ 0,02, n.s., not significant