Table 2. Staining methods times in hystoresin to histological analyses.
| Staining method | Sections (µm) | Reagents | Time | Wash (times) | Temperature (°C) |
|---|---|---|---|---|---|
| Toluidine blue | 3 | Toluidine blue | 0:01:30 | – | room |
| Destilled water | – | 1 | room | ||
| Hematoxylin-Eosin | 3 | Hematoxylin | 0:15:00 | – | 39 |
| Tap water | 0:03:00 | – | room | ||
| Destilled water | – | 1 | room | ||
| Eosina | 0:05:00 | – | 39 | ||
| Destilled water | 0:02:00 | 3 | room | ||
| Alcohol 70% | – | 1 | room | ||
| Destilled water | – | 1 | room | ||
| Periodic acid-Schiff | 5–7 | Periodic acid | 0:20:00 | – | 39 |
| Destilled water | 0:02:00 | 3 | room | ||
| Schiff | 1:00:00 | – | room | ||
| Tap water | 0:05:00 | – | room | ||
| Destilled water | – | 1 | room | ||
| Alcian blue pH 2.5 | 5–7 | Acetic acid 3% | 0:06:00 | room | |
| Alcian blue pH 2.5 | 2:00:00 | – | 39 | ||
| Tap water | 0:03:00 | room | |||
| Destilled water | – | 1 | room | ||
| Mercury-bromophenol blue | 5–7 | Mercury-bromophenol blue | 1:00:00 | – | room |
| Acetic acid 0.5% | 0:10:00 | – | room | ||
| Buffer solution | – | 1 | room | ||
| Naphthol yellow S | 5–7 | Naphthol yellow S | 1:00:00 | – | room |
| Acetic acid 1.0% | 0:10:00 | – | room | ||
| Tertiary butyl alcohol | – | 1 | room |
Notes.
–, not applicable data.