Table 1.

Sensitivity and specificity of FISH by and IHC for the detection of ALK rearrangement in NSCLC cancer by technique and probe

Screening Test		Sensitivity (%)	Specificity (%)
FISH	Number of tumor areas examined under high-power microscopy fields
	2	98.6	96.6
	3	99.3	100
	4	100	100
IHC	Probe and Detection System
	5A4 by Novocastra with ADVANCE [25]	100	87.5
	5A4 by Nichirei with Histofine [25]	100	62.5
	D5F3 by Cell Signaling with ADVANCE [25]	100	75
	ALK1 by DAKO with FLEX [25]	66	100
	ALK1 by DAKO with ADVANCE [25]	66	87.5
	5A4 by Novocastra with i-view [27]	100	95.8
	5A4 by Novocastra with BenchMark XT [28]	96	100
	D5F3 by Cell Signaling BenchMark XT [28]	96	100
	5A4 by Novocastra with Bond-MAX [28]	96	100
	5A4 by Abcam with Bond-MAX and UltraView Universal DAB [31]	69	99.3
	5A4 by Novocastra with Envision Plus [76]	93	100
	5A4 by Abcam with Benchmark Ventana [77]	95	100
	D5F3 by Ventana with Optiview [78]	98	100
	5A4 by Novocastra with Optiview [78]	98	100

Abbreviations: FISH, fluorescence in situ hybridization; IHC, immunohistochemistry