Figure 5. PMA induced changes in MT1-MMP function.

A. Primary aortic fibroblasts from normal mice (n=4) were treated with or without PMA, in the presence or absence of Röttlerin for 18 h (n=4 each treatment group). Cell homogenates were fractionated by gel electrophoresis and immunoblotted for latent and active MMP2. Results are expressed as a mean percent change in each cell line from its respective steady-state control (vehicle treated; dotted line). Individual data points in each group overlay box plots. The box defines the 25^th–75^th interquartile range, and both the median (red line) and mean (blue line) values are shown within the box. * p<0.05 versus control (ANOVA with Tukey’s wsd). B. Primary aortic fibroblasts from normal mice (n=4) were treated with or without PMA (30 min), in the presence or absence of Röttlerin (3 h pre-incubation, plus 30 min concurrent; n=4 each treatment group). Cell homogenates were fractionated by gel electrophoresis and immunoblotted for phosphor-Smad-2. Results are expressed as a mean percent change in each cell line from its respective steady-state control (vehicle treated; dotted line). Individual data points in each group overlay box plots. The box defines the 25^th–75^th interquartile range, and both the median (red line) and mean (orange line) values are shown within the box. * p<0.05 versus control, # p<0.05 versus PMA (ANOVA with Tukey’s wsd).