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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Brain Behav Immun. 2016 Nov 14;61:127–136. doi: 10.1016/j.bbi.2016.11.012

Figure 4. Achl knock-down in neurons protects flies against bacterial infection.

Figure 4

(A). Achl knock-down flies have a higher survival rate after S. aureus infection. Data are the combined results of at least four biological replicates. N > 50 for infected flies and > 30 for injury only flies. *** = p < 0.005 Log rank test.

(B). Achl knock-down flies have a better survival rate after P. aeruginosa infection. Data are the combined results of at least four biological repeats. N > 50 for infected flies and > 30 for injury only flies. *** = p < 0.005 Log rank test.

(C). Achl knock-down flies have decreased bacterial colony formation after bacterial infection. N > 30 for infected flies and > 20 for injury only flies. ***= p < 0.005 Students’ T-test. Mean ± SEM of Control-P. aeruginosa: 4.898e+006 ± 1.105e+006; RNAi-P. aeruginosa: 606616 ± 159290; Control-S. aureus: 7.687e+006 ± 1.301e+006; RNAi-S. aureus: 324859 ± 110479.

(D). Expression of IM1 upon infection by qPCR assay (N = 2 biological replicates of 6–8 flies apiece; Error bars are +/− SEM). 0 hour expression level is higher in RNAi than control flies; 24 hours expression level is similar in RNAi and control flies for each inoculation condition. All qPCR data were normalized with control 0 hour injury only data.