Figure 2. CTC-like cells detected in healthy volunteers.

Representative immunofluorescence (IF) examples (100× magnification) of traditional and non-traditional CTC-like cells in healthy volunteer samples. Traditional (DAPI⁺/CD45⁻/cytokeratin [CK]⁺/abnormal morphology), CK⁻ (DAPI⁺/CD45⁻/CK⁻/AR⁺/abnormal morphology), and apoptotic (DAPI⁺/CD45⁻/CK⁺/nuclear disintegration/abnormal morphology) CTC-like cells from healthy volunteers are shown. Blue = DAPI, green = CD45, Red = CK, White = AR.