Fig. 1.

a Effects of media on the cell growth and SrtA expression. Light gray and dark gray bars represent the SrtA activity and biomass in mediums of LB, TB, SOB, SB and 2×YT. b Effects of addition of defined compositions in media on the expression of SrtA. Light gray and dark gray bars represent the SrtA activity and biomass in the TB medium with addition of glycerol, MOPS, and PBS buffer. The TB medium without glycerol and PBS buffer was used as the control. c Effects of various carbon sources on the expression of SrtA. Light gray and dark gray bars represent the SrtA activity and biomass in the medium with various carbon sources of glucose, fructose, galactose, maltose, lactose, sucrose, glycerol, dextrin, and starch. d Effects of nitrogen sources on the expression of SrtA. Light gray and dark gray bars represent the SrtA activity and biomass in the medium with various nitrogen sources of tryptone, beef extract, soya peptone, fishmeal, casein, urea, ammonium chloride, ammonium sulfate, and ammonium citrate