Fig. 5.

Validation of the optimal conditions for SrtA expression. The LB medium was used as the control at 37 °C for 4-h fermentation, while the optimized media (0.48 g/L glycerol, 1.37 g/L tryptone, 0.51 g/L yeast extract, MOPS 0.5 g/L, PBS buffer 180 mL/L) were operated at 30 °C for 8 h. a The SrtA activity in the control and optimized media at 7-L fermentor level; b the concentration of purified SrtA in the control and optimized media at 7-L fermentor level