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Effect of insulin on the types and numbers of follicles (mean ± SEM) in ovarian cortical pieces isolated from late-term bovine fetuses. Cortical pieces were cultured for 0 (Day 0; black bar) or 4 (white bars) days in medium supplemented with ITS+ (contains insulin) or with TS+ (identical to ITS+ but without insulin). Within each panel, means (bars) with no common letters (a, b, c) differ significantly (P < 0.05; n = 5 fetuses).
