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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Hepatol. 2016 Nov 18;66(3):601–609. doi: 10.1016/j.jhep.2016.11.004

Fig. 5. Aging exacerbates long-term (8wks)-plus-multiple binges-induced liver fibrosis.

Fig. 5

Young and middle-aged mice were treated with chronic (8wks)-plus-multiple binges as described in Fig. 2. (A) Representative images of Sirius Red staining at a magnification of 200x. Sirius Red positive area was quantified. (B) Western blot analysis of hepatic expression levels of α-SMA. (C) Quantitative real-time PCR analysis of fibrosis related genes. Values represent means ± SEM (n= 4–8). *P< 0.05, **P<0.01.