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. 2017 Feb 20;8:244. doi: 10.3389/fpls.2017.00244

FIGURE 6.

FIGURE 6

Dithiothreitol blocked the effects of melatonin on (A) VDE activity, (B) the de-epoxidation state of xanthophyll pigments and (C) the induction of NPQ in the leaves of tomato seedlings following exposure to light during chilling. Leaves of tomato (Solanum lycopersicum L. cv. Micro-Tom) seedlings at the three-leaf stage were pretreated with 100 μmol melatonin (MT) one time a day for 3 days. At the end of light cycle at 18:00 on day 3, they were exposed to chilling (4°C) for 10 h in the dark, then in the light (400 μmol m-2 s-1) next morning for another 6 h. Tomato seedlings were infiltrated with either 5 mM dithiothreitol (DTT) or with water from stem 2 h before they were exposed to light. Samples were collected at 0, 5, 10, 30, and 60 min following light exposure. The values presented are mean ± SD (n = 3). Different letters indicate significant differences at P < 0.05 among treatments.