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Electron microscopy (EM) immunogold labeling of Syt1 (A–C): The signal over the plasma membrane (PM) of freshly divided cells (A); the labeling on symbiosome membrane, note the contact with ER (B); (C) The signal on symbiosome membrane and ER. Gold granules are pointed by arrowheads. CW, cell wall; ER, endoplasmic reticulum; S, symbiosome. Bars: (A) = 200 nm, (B) = 500 nm, (C) = 200 nm
