Figure 4.

Protein Expression Changes during Neuronal Differentiation

(A) Schematic overview of neurogenesis, illustrating the distinct developmental stages and representative transcription factors involved.

(B) Expression profiles for representative transcription factors including the most downregulated protein in this dataset, Sox11.

(C–F) Expression profiles of selected presynaptic proteins (C), postsynaptic proteins (D), cell-adhesion proteins (E) and proteins with no relative expression change over the developmental stages (F).

(G) Change in the protein expression during neuronal development shown by western blot analysis from cultured hippocampal neurons, embryonic tissue (Hip.(E)), and adult brain tissue (Hip.(A)).

(H and I) Graphs showing the quantifications of relative intensities of the western blot analysis in (G), shown in (H), and control proteins, shown in (I). n = 3 experiments per condition. Error bars indicate mean ± SEM.

In (B)–(F), error bars represent SD.