Figure 7.

NCAM1 and Its Role in Actin Cytoskeleton Stabilization

(A) Selected candidates of putative NCAM1 interactors identified in young and adult rat brains by AP-MS experiments (see Table S6 for the complete list of proteins). SAINT probability cutoff > 0.75 corresponds to an average FDR of 0.1. The p values and spectral counts are graphically represented by colors and spheres, respectively.

(B) Network analysis on selected NCAM180-binding proteins linked to actin cytoskeleton. Edge color coding: blue, co-localization; orange, predicted interaction; green, shared protein domain; purple, co-expression.

(C) Heatmap and hierarchical clustering of the expression profiles of selected NCAM1-interacting proteins.

(D) Schematic representation of NCAM180 associated with actin-stabilizing proteins.

(E) Representative images of primary hippocampal neurons co-transfected with indicated constructs (DIV1–DIV7) and treated 24 hr later with DMSO or 10 nM jasplakinolide. Scale bar, 50 μm.

(F and G) Quantification of axon (F) and dendrite (G) morphological parameters.

Error bars indicate mean ± SEM. ^∗p < 0.5, t test; ^∗∗p < 0.01, t test.

See also Table S6.