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. 2017 Feb 13;8:14421. doi: 10.1038/ncomms14421

Figure 6. Loss-of-function assay of candidate lncRNAs regulating cell migration.

Figure 6

Transwell migration assays were conducted to test the effects of siRNA-mediated RNAi of candidate lncRNAs in three liver cancer cell lines: HepG2, SMMC-7721 and HCCLM9. (a) The value in the heatmap is the fold-change (P value<0.05) of the transwell cell numbers for knockdown cells over those of control cells. All results are expressed as the mean derived from three independent experiments. The unpaired Student's t-test (two-tailed) was used for comparisons of two groups. Seven of the ten candidate lncRNAs were metastasis-associated lncRNAs that were recurrently deregulated in PVTTs (in bold font). *P value<0.05, **P value<0.01, ***P value<0.001, t-test, n=3. (b) RNAi was validated by qRT-PCR. Examples of migration phenotype: transwell cells (DAPI staining) (c) (Scale bar, 500 μm) and their counts (d). Error bars represent the s.d. of three experiment replicates.