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. 2017 Feb 13;8:14340. doi: 10.1038/ncomms14340

Figure 2. Generation of NDV-expressing murine ICOSL (NDV-ICOSL).

Figure 2

(a) NDV genomic construct scheme. (b) Expression of mICOSL in human melanoma cell lines 24 h after infection. Representative histograms (left) and median fluorescence intensity (MFI) bar graphs (right) are shown. (c) Expression of mICOSL on the surface of murine tumour cells 24 h after infection. Representative histograms from B16-F10 and MB49 cells (left), and MFI bar graphs (right) are shown. (d) Replication of parental NDV (NDV-WT) and NDV-ICOSL in B16-F10 cells. (e) In vitro lysis of B16-F10 cells by NDV-WT and NDV-ICOSL. (f) Expression of ICOSL in tumour cells 24 h after NDV-ICOSL treatment. Representative flow cytometry plots gated on CD45− cells (left) and summary graph (right) are shown. ***P<0.001, ****P<0.0001, NS, non-significant. (a–e) Representative data from three experiments with three samples per group. (f) Representative data from two experiments with five animals per group. Data with error bars represent mean±s.e.m. Two-tailed unpaired Student's t test was used for figures 2b,c, and one way ANOVA was used for 2f.