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. 2017 Feb 16;8:14395. doi: 10.1038/ncomms14395

Figure 5. Anti-miR-17 treatment reduces proliferation and cyst growth in in vitro models of human ADPKD.

Figure 5

Primary cyst epithelial cultures derived from kidneys of ADPKD patients were transfected with anti-miR-17 (dose: 3 nM, 10 nM or 30 nM) or three different control oligonucleotides (dose: 30 nM, control oligo1, 2 and 3). These cells were then cultured to measure proliferation or in vitro cyst formation (3-Dimension Matrigel). (a) Data for proliferation (n=5 for each treatment and dose) and (b) representative images and (c) quantification of cyst formation (n=3 for each treatment and dose) using primary cultures derived from ADPKD Donor 4 are shown. Anti-miR-17 reduced proliferation and cyst count in a dose-dependent manner. Data from the other ADPKD Donors can be found in the Supplementary Fig. 6. Error bars represent s.e.m. *indicates P<0.05, **indicates P<0.01, ***indicates P<0.005 and ****indicates P<0.001. One-way ANOVA, Tukey's multiple comparisons test.