Figure 3. In vivo evaluation of LbCpf1–crRNA3 activity at 13 potential off-target sites in the genome.

The indel frequencies (%) at 13 candidate off-target (OT) sites (with up to 4 bp mismatches relative to the crRNA3) were measured and validated in LbCpf1–crRNA3-delivered soybean protoplasts by targeted deep sequencing. No mutations were detected at any of the 13 candidate loci. Error bars represent s.d. (n=2). Blue, mismatched nucleotide bases; Red, PAM sequences.