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. 2017 Feb 16;8:14406. doi: 10.1038/ncomms14406

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Copyright © 2017, The Author(s)

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(a) Insertion of the template DNA used for crRNA in vitro transcription at the target site. (b) Indel frequency (%, Log10 scale at Y axis) and (c) indel patterns at the target sites after delivery of Cpf1 protein and chemically synthesized crRNA3 (CS-crRNA3) into soybean protoplasts. Error bars represent s.d. (n=2). blue, crRNA base-pairing site; green, inserted sequence derived from crRNA template; MT, mutated sequence; red, PAM sequence; WT, wild-type sequence.