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. 2017 Feb 15;8:14228. doi: 10.1038/ncomms14228

Figure 4. Overexpression of Dub3 induces EMT.

Figure 4

(a) WT- or CS-Dub3 was expressed in MCF7 and T47D cells. A rescue experiment with knockdown of Snail1 expression in WT-Dub3 expressing cells was also performed. The level of Snail1, Dub3, E-cadherin and ERα was analysed by western blot. (b) WT- or CS-Dub3 was expressed in MCF7 cells. A rescue experiment with knockdown of Snail1 expression in WT-Dub3 expressing cells was also performed. Morphologic changes indicative of EMT are shown in the phase contrast images; expression of E-cadherin, ERα and Dub3 was assessed by IF staining. Nuclei were visualized with DAPI (blue). Scale bars, 20 μm. (c,d) WT- or CS-Dub3 was expressed in MCF7 cells. A rescue experiment with knockdown of Snail1 expression in WT-Dub3 expressing cells was also performed. The mRNA levels of epithelial, mesenchymal (c), luminal, and basal (d) markers were quantitated by real-time PCR. Data are shown as mean±s.d. of two separate experiments in triplicates. (e) Boyden chamber migration assay of modified MCF7 and T47D cells, as described in a. Data are presented as mean±s.e.m. (f) Boyden chamber invasion assay of modified MCF7 and T47D cells, as described in a. Data are presented as mean±s.e.m.