Table 1.
Summary of the results obtained for editing of AvrXa7 and TalC EBEs in O. sativa cv. Kitaake using TALENs
| AvrXa7 EBE | TalC EBE | |
|---|---|---|
| Number of T0 plants studied | 135 | 171 |
| Number of T0 plants carrying both transgenes | 113 | 154 |
| Number of T0 edited plants retrieved | 58 | 46 |
| Edition efficiency (edited vs. carrying both transgenes) | 51% | 30% |
| Number of independent T0 lines retrieved | 30 | 23 |
| Number of distinct edited alleles and classification (deletions/insertions/combined events) | 41 (27/3/11) | 26 (19/2/5) |
| Range of the deletions | −1 to −51 bp | −3 to −24 bp |
| Range of the insertions | +1 to +23 bp | +1 to +10 bp |
| Number of independent T0 lines genotyped at the T1 generation | 11 | 10 |
| Number of independent edited alleles available at the homozygous stage (deletions/insertions/combined events) | 19 (15/1/3)a | 16 (13/2/1)b |
| Number of transgene‐free T1 lines carrying a unique edited allele at the homozygous stage | 10 | 5 |