Figure 4. Plin5 deficiency reduced lipid storage and elevated the cytosolic fatty acid levels in myocardium with I/R injury.

(A) Oil Red O staining showed that Plin5 deficiency led to reduced cardiac lipid accumulation in mice with either sham or I/R surgery (n = 4). Scale bar = 20 μm. (B–D) Quantitative analysis of serum TG levels (B), myocardial TG contents (C), and FFA contents (D) (n = 4). Serum TG levels were obviously increased after I/R injury, but no difference was observed between wild-type and Plin5-null mice. (C) The amounts of TG storage decreased slightly, whereas the levels of intracellular FFA (D) increased in Plin5-deficient myocardium. The columns and errors bars represent the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. FFA, free fatty acid.