Figure 2. PT-Mangiferin reduced generation of ROS and inhibited translocation of NF-κB in RAW 264.7 cells.

(A) RAW 264.7 cells were treated with PBS, LPS (1 μg/ml) followed by treatment with Celecoxib (20 μM) or different doses of PT-Mangiferin (20, 40 μM) and cells were kept for 16 h at 37 °C/5% CO₂. ROS levels were measured by Flow cytometry. N-Acetyl Cysteine, a ROS inhibitor was used as a positive control. (B) Nuclear extract of the cells with above treatments were prepared and analyzed for translocation of p50 and p65 sub units using specific antibodies as described in materials and methods. Histone 3(H3) was used as internal control. The gels were run under same experimental conditions. PBS- Phosphate buffer saline, LPS-Lipopolysaccharide (1 μg/ml), CXB- Celecoxib (20 μM), MF-20- PT-Mangiferin (20 μM), MF-40- PT-Mangiferin (40 μM).