Figure 4. PT-Mangiferin reduced the infiltration of monocytes and neutrophils and production of pro-inflammatory cytokines in the air pouch.

Animals from different treatment groups were killed, air pouch were exposed and exudates were recovered with 500 μl PBS as described in materials and methods. (A) Volume of exudate recovered from each group. (B) Total cells in the exudates from each group as determined by counting. (C) Percent of each cell type in the exudates as analyzed by Flow cytometry. (D) FACS plots showing neutrophil population in each group. (E) MPO analysis. (F) Analysis of cytokines (IL-6, TNF-α, IL-1β) in the inflammatory exudates by ELISA. PBS- Phosphate buffer saline, CGN-Carrageenan (1.5%, 0.5 ml), CXB- Celecoxib (10 mg/kg body weight), MF-10- PT-Mangiferin at 10 mg/kg body weight, MF-20- PT-Mangiferin at 20 mg/kg body weight.