Figure 5. Local injection of Fe(Salen) nanoparticles generates heat upon exposure to AMF, and this heat enhances the anti-tumor effect in the GB mouse model.

(a) Illustration of a mouse in the coil of the AMF generator. (b) Magnetic flux density distribution from the simulation (AMF: 280 kHz and 335.4 Arms). Colors indicate the magnetic flux density. The distance between the tumor and the edge of coil, and the estimated magnetic flux density is shown. (c) Relationship between the magnetic flux density and the distance of the tumor from the edges of the coil during AMF exposure (280 kHz and 335.4 Arms). (d) Representative thermography image at the tumor site (left) after injection of Fe(Salen) nanoparticle suspension before (middle)/after AMF exposure (right). (e) Temperature change at the rectum and the injection site of Fe(Salen) suspension with saline or saline upon AMF exposure. Either Fe(Salen) nanoparticle suspension or saline was injected into the tumor in mice, followed by exposure to AMF. Note that the local temperature was time-dependently increased to a greater degree with Fe(Salen) (n = 6, ****p < 0.0001). (f) Treatment schedule for GB mouse experiment. (g) Representative pictures of mouse leg tumor in each treatment group. (h) IVIS images of mouse leg tumor in each treatment group at Day 0 (upper) and Day 28 (lower). (i) Regression rate based on manual measurement of tumor volume changes in mouse leg. The red line indicates the ratio in the Fe(Salen) with AMF exposure group (n = 6, ns, not significant, ****p < 0.0001). (j) Regression rate based on photon flux measurement of tumor volume changes in mouse leg. The red line indicates the ratio in the Fe(Salen) with AMF exposure group (n = 6, ns, not significant, *p < 0.05, ***p < 0.001).