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. 2017 Feb 20;7:43060. doi: 10.1038/srep43060

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(a) the changes in the titer of anti-PIT-1 antibody. The titer was determined by immunoblotting with a serial dilution of sera. The results of immunoblotting are shown in Supplementary Figure. (b,c) (b) Images of the results of enzyme-linked immunospot (ELISpot) assay. (c) Quantitative analysis of the ratio of the spots toward PIT-1 in accordance with positive control.