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. 2017 Feb 20;7:41095. doi: 10.1038/srep41095

Figure 5. Editing slows Kvβ1.1-induced inactivation kinetics of I407M and V408A channels.

Figure 5

(a,c) Representative β-inactivation traces, depicting whole-cell K+ currents, were recorded from oocytes co-expressing the Kvβ1.1 subunit and either the (a) I407M or (c) V408A channel, in the non-edited (N) or edited (E) isoform. Test potentials were elicited in 10 mV voltage steps from 10 to 80 mV, from a holding potential of −80 mV. (b,d) Inactivation kinetics were measured by fitting single exponential curves to the test pulse currents, to determine the associated τ value (mean ± SEM, n = 3–6 oocytes). (b) I407M E channels were significantly slower to inactivate than I407M N channels at every voltage (p ≤ 0.0001) and both I407M N and I407M E channels were slower than WT N and WT E channels, respectively, at every voltage (p ≤ 0.0001). (d) V408A E channels were significantly slower than V408A N channels from 10 to 50 mV (0.05 > p ≥ 0.0005). V408A E channels were slower than WT E channels from 10 to 60 mV (0.05 > p ≥ 0.0001). V408A N channels were significantly slower than WT N channels at all voltages (p ≤ 0.0001). Small error bars were obscured by the data symbols in some cases.