Table 1. Voltage-dependence of activation.

	V1/2 (mV)			k (mV)
WT N	−31.6 ± 1.7			16.0 ± 0.9
WT E	−34.5 ± 2.3			12.3 ± 1.4
V404I N	−9.5 ± 1.4	****	]+++	14.7 ± 1.1
V404I E	−20.3 ± 1.5	***		14.2 ± 0.9
I407M N	−1.9 ± 0.8	****		10.7 ± 0.2	***	]++
I407M E	2.5 ± 1.1	****		9.4 ± 0.2
V408A N	−29.4 ± 0.9			9.1 ± 1.0	***
V408A E	−23.6 ± 3.4			8.1 ± 0.4

Voltage-dependence of activation was determined by fitting data to a Boltzmann function, equation (2), to determine the midpoint of channel activation (V_1/2) and relative voltage sensitivity (k). All data are represented as mean ± SEM, n = 4–8 oocytes for each channel type. Edited (E) and non-edited (N) isoforms of the mutant channels were compared to WT E and WT N channels, respectively: ***p ≤ 0.001; ****p < 0.0001. All types of N channels were compared to their respective E channels: ⁺⁺p ≤ 0.005; ⁺⁺⁺p ≤ 0.001. Due to multiple comparisons, significance was set at p ≤ 0.005.