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. 2017 Feb 20;7:42737. doi: 10.1038/srep42737

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(a) Optimization of temperature of LAMP. M GeneRuler^TM 100 bp Plus DNA Ladder; lanes 57 °C–69 °C indicated the reaction temperatures of LAMP. (b) Optimization of time duration of LAMP. M GeneRulerTM 100 bp Plus DNA Ladder; lanes 15 m–90 m indicated the reaction time duration of LAMP. All the products were detected on the basis of 2% agarose gel electrophoresis.