Table 1. Details of thee fungal isolates, plant and soil samples used in the LAMP detection assay.
Samples | Collection site | State | LAMP detection |
Conventional PCR |
||
---|---|---|---|---|---|---|
Florescence | Agarose gel | RB_F3/RB_B3 | ITS1/ITS4 | |||
R. bataticola isolates (chickpea) | ||||||
RB1 | Kanpur | Uttar Pradesh | + | + | + | + |
RB2 | Coimbatore | Tamil Nadu | + | + | + | + |
RB3 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
RB4 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
RB5 | ICRISAT, BP 4 | Andhra Pradesh | + | + | + | + |
RB6 | ICRISAT, BP 10 | Andhra Pradesh | + | + | + | + |
RB7 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
RB8 | ICRISAT, BUS 03 | Andhra Pradesh | + | + | + | + |
RB9 | ICRISAT, BR 05 | Andhra Pradesh | + | + | + | + |
RB10 | ICRISAT, BIL 02 | Andhra Pradesh | + | + | + | + |
RB11 | ICRISAT, BIL 02 | Andhra Pradesh | + | + | + | + |
RB12 | Pati | Andhra Pradesh | + | + | + | + |
RB13 | ICRISAT, BP 02 | Andhra Pradesh | + | + | + | + |
RB14 | Jodhpur | Madhya Pradesh | + | + | + | + |
RB15 | Jabalpur | Madhya Pradesh | + | + | + | + |
RB16 | Delhi | Delhi | + | + | + | + |
RB17 | Damoh 1 | Madhya Pradesh | + | + | + | + |
RB18 | Damoh 2 | Madhya Pradesh | + | + | + | + |
RB19 | Damoh 3 | Madhya Pradesh | + | + | + | + |
RB20 | Damoh 4 | Madhya Pradesh | + | + | + | + |
RB21 | Damoh 5 | Madhya Pradesh | + | + | + | + |
RB22 | Damoh 6 | Madhya Pradesh | + | + | + | + |
RB23 | Damoh 7 | Madhya Pradesh | + | + | + | + |
RB24 | ICRISAT, BUS 04 | Andhra Pradesh | + | + | + | + |
RB25 | ICRISAT, BUS 07 | Andhra Pradesh | + | + | + | + |
RB26 | ICRISAT, BIL 02 | Andhra Pradesh | + | + | + | + |
RB27 | ICRISAT, BUS 07 | Andhra Pradesh | + | + | + | + |
RB28 | ICRISAT, BR 04H | Andhra Pradesh | + | + | + | + |
RB29 | ICRISAT, BUS 03 | Andhra Pradesh | + | + | + | + |
RB30 | ICRISAT, BIL 02D | Andhra Pradesh | + | + | + | + |
RB31 | ICRISAT, BUS 03 | Andhra Pradesh | + | + | + | + |
RB32 | ICRISAT, BIL 05 | Andhra Pradesh | + | + | + | + |
RB33 | ICRISAT, BP 04 | Andhra Pradesh | + | + | + | + |
RB34 | ICRISAT, BR 05D | Andhra Pradesh | + | + | + | + |
RB35 | ICRISAT, BUS 03 | Andhra Pradesh | + | + | + | + |
RB36 | ICRISAT, BR 04F | Andhra Pradesh | + | + | + | + |
RB37 | ICRISAT, BP 04 | Andhra Pradesh | + | + | + | + |
RB38 | ICRISAT, BR 04F | Andhra Pradesh | + | + | + | + |
RB39 | ICRISAT, BM 14 | Andhra Pradesh | + | + | + | + |
RB40 | ICRISAT, BR 04 | Andhra Pradesh | + | + | + | + |
RB41 | ICRISAT, BP 04 | Andhra Pradesh | + | + | + | + |
RB42 | ICRISAT, BR 04F | Andhra Pradesh | + | + | + | + |
RB43 | ICRISAT, BW 01A | Andhra Pradesh | + | + | + | + |
RB44 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
RB45 | ICRISAT, BW 03 | Andhra Pradesh | + | + | + | + |
RB46 | ICRISAT, BW 04 | Andhra Pradesh | + | + | + | + |
RB47 | ICRISAT, BR 04I | Andhra Pradesh | + | + | + | + |
RB48 | ICRISAT, BR 04H | Andhra Pradesh | + | + | + | + |
RB49 | Jabalpur 1 | Madhya Pradesh | + | + | + | + |
RB50 | Jabalpur 5 | Madhya Pradesh | + | + | + | + |
RB51 | Jabalpur 6 | Madhya Pradesh | + | + | + | + |
RB52 | Jabalpur 8 | Madhya Pradesh | + | + | + | + |
RB53 | Brampuri, Damoh 8 | Madhya Pradesh | + | + | + | + |
RB54 | Brampuri, Damoh 9 | Madhya Pradesh | + | + | + | + |
RB55 | Katni | Madhya Pradesh | + | + | + | + |
RB56 | JNKVV, Jabalpur | Madhya Pradesh | + | + | + | + |
RB57 | Rewa | Madhya Pradesh | + | + | + | + |
RB58 | Bachara. Satna | Madhya Pradesh | + | + | + | + |
RB59 | IIPR, Kanpur | Uttar Pradesh | + | + | + | + |
RB60 | ICRISAT, BIL 02 | Andhra Pradesh | + | + | + | + |
RB61 | Dhaulakuan | Himachal Pradesh | + | + | + | + |
RB62 | Ramnagar, Pantnagar | Uttarakhand | + | + | + | + |
RB63 | BAU, Ranchi | Jharkhand | + | + | + | + |
RB64 | ICRISAT, BP 02C | Andhra Pradesh | + | + | + | + |
RB65 | ICRISAT, BP 15 | Andhra Pradesh | + | + | + | + |
RB66 | ICRISAT, BP 03B | Andhra Pradesh | + | + | + | + |
RB67 | ICRISAT, BP 03C | Andhra Pradesh | + | + | + | + |
RB68 | ICRISAT, BP 08A | Andhra Pradesh | + | + | + | + |
RB69 | ICRISAT, BP 08B | Andhra Pradesh | + | + | + | + |
RB70 | ICRISAT, BR 05D | Andhra Pradesh | + | + | + | + |
RB71 | ICRISAT, BR 05B | Andhra Pradesh | + | + | + | + |
RB72 | ICRISAT, BL 04A | Andhra Pradesh | + | + | + | + |
RB73 | ICRISAT, BL0 4 | Andhra Pradesh | + | + | + | + |
RB74 | ICRISAT, BM 13 | Andhra Pradesh | + | + | + | + |
RB75 | ICRISAT, BM 13 | Andhra Pradesh | + | + | + | + |
RB76 | ICRISAT, BM 08C | Andhra Pradesh | + | + | + | + |
RB77 | ICRISAT, BW 02A | Andhra Pradesh | + | + | + | + |
RB78 | ICRISAT, BW 02B | Andhra Pradesh | + | + | + | + |
RB79 | ICRISAT, BW 02C | Andhra Pradesh | + | + | + | + |
RB80 | ICRISAT, BW 04A | Andhra Pradesh | + | + | + | + |
RB81 | ICRISAT, BW 05A | Andhra Pradesh | + | + | + | + |
RB82 | ICRISAT, BW 05B | Andhra Pradesh | + | + | + | + |
RB83 | ICRISAT, BW 08 | Andhra Pradesh | + | + | + | + |
RB84 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
RB85 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
RB86 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
RB87 | ICRISAT, BIL 03C | Andhra Pradesh | + | + | + | + |
RB88 | ICRISAT, BIL 03 | Andhra Pradesh | + | + | + | + |
RB89 | ICRISAT, BIL 04 | Andhra Pradesh | + | + | + | + |
RB90 | ICRISAT, BIL 05B | Andhra Pradesh | + | + | + | + |
RB91 | ICRISAT, BIL 05C | Andhra Pradesh | + | + | + | + |
RB92 | ICRISAT, BIL 05C | Andhra Pradesh | + | + | + | + |
RB93 | ICRISAT, JM 08B | Andhra Pradesh | + | + | + | + |
RB94 | ICRISAT, BR 05C | Andhra Pradesh | + | + | + | + |
DRR infected chickpea plants (field) | ||||||
Sample 1 | ICRISAT, BIL 02 | Andhra Pradesh | + | + | + | + |
Sample 2 | ICRISAT, BW 08 | Andhra Pradesh | + | + | + | + |
Sample 3 | ICRISAT, BM 13 | Andhra Pradesh | + | + | + | + |
Sample 4 | ICRISAT, BP 15 | Andhra Pradesh | + | + | + | + |
Sample 5 | ICRISAT, BIL 01 | Andhra Pradesh | + | + | + | + |
Healthy | ICRISAT, BIL 02 | Andhra Pradesh | − | − | − | − |
DRR infected chickpea plants (greenhouse) | ||||||
Sample 1 | Experimental sample | ICRISAT | + | + | + | + |
Sample 2 | Experimental sample | ICRISAT | + | + | + | + |
Sample 3 | Experimental sample | ICRISAT | + | + | + | + |
Sample 4 | Experimental sample | ICRISAT | + | + | + | + |
Healthy | Experimental sample | ICRISAT | − | − | − | − |
Sick soil (DRR) | ||||||
Rhizospheric black soil | ICRISAT, BP 04 | Andhra Pradesh | + | + | + | MBa |
Rhizospheric red soil | ICRISAT, RL17 | Andhra Pradesh | + | + | + | MB |
Sick soil (non-rhizospheric) | Experimental sick plot | ICRISAT | + | + | + | + |
Other fungal pathogens | ||||||
F. oxysporum f. sp. ciceris (chickpea) | In vitro culture | ICRISAT | − | − | − | + |
R. solani (chickpea) | In vitro culture | ICRISAT | − | − | − | + |
S. rolfsii (chickpea) | In vitro culture | ICRISAT | − | − | − | + |
F. solani (chickpea) | In vitro culture | ICRISAT | − | − | − | + |
F. udum (pigeonpea) | In vitro culture | ICRISAT | − | − | − | + |
P. cajani (pigeonpea) | In vitro culture | ICRISAT | − | − | − | + |
aMultiple bands.