Table 1. Cross-platform analysis of the HRM assay.
| Virus genotype | Real-Time PCR machines with Tm values | |||
|---|---|---|---|---|
| CFX96 (Bio-Rad) | LC480II (Roche) | QS6 (Life tech.) | RG-Q (Qiagen) | |
| CPXV | 72.20–72.40 | 72.70–72.75 | 72.75–72.88 | 73.44–73.47 |
| CMLV | 73.00–73.20 | 73.38–73.80 | 73.38–73.51 | 74.10–74.13 |
| GTPV | 75.60–75.80 | 76.49–76.53 | 76.38–76.48 | 77.14–77.20 |
| SPPV | 76.20–76.40 | 76.98–77.05 | 76.93–76.98 | 77.70–77.72 |
| LSDV | 77.20–77.40 | 78.00–78.03 | 77.84–77.96 | 78.70–78.73 |
| ORFV | 80.20–80.40 | 80.62–80.65 | 80.36–80.58 | 81.36–81.39 |
| PCPV | 81.20–81.40 | 82.08–82.10 | 81.82–81.95 | 82.70–82.71 |
| BPSV | 81.60–81.80 | 82.42–82.45 | 82.05–82.23 | 82.96–83.02 |
Different real-time PCR instruments used for assay evaluation with their respective amplicon melting temperature values indicated for the targeted eight poxvirus species.